DAMNDPATHS - Elucidation of common transcriptional targets in vulnerable dopamine, motor neuron and frontotemporal dementia disease pathways

JPND

Project goal

The goal of the DAMNDPATHS project is to identify shared mechanisms or pathways leading to selective neuronal cell death in a group of clinically distinct neurodegenerative diseases. We will start with three devastating motor neuron diseases: amyotrophic lateral sclerosis (ALS), spinal muscular atrophy (SMA) and spinobulbar muscular atrophy (SBMA) - - all characterized by the loss of neurons that control voluntary muscles. Genetic causes of these diseases are different, but the patterns of neuronal degeneration are similar.

We will use RNA sequencing technology to identify genes that are activated in either vulnerable or degeneration-resistant neurons during disease progression. Targets will be verified in post mortem tissues from ALS patients, as well as Frontotemporal dementia and Parkinson’s Disease, to evaluate cross-disease relevance of the genes. We will then use cellular disease models based on patient-specific neurons from induced pluripotent stem cells, to examine the function of candidate genes in neuronal vulnerability or resistance. Finally, we will examine the ability of selected genes to modify disease progression in vivo in mouse models.

We are confident that these complementary approaches will lead us to discover key pathways at a stage of disease that may still be reversible, thus opening new avenues for the development of disease-modifying agents that can alleviate the burden of these devastating diseases.

 

Project Partners:

Associate professor Eva Hedlund, Karolinska Instituet, Stockholm, Sweden  (coordinator)

Associate professor Eva Hedlund (coordinator)

http://ki.se/en/neuro/hedlund-laboratory

http://ki.se/en/people/evahed

 

 

Associate professor Rosario Sanchez-Pernaute, Fundacion Inbiomed, San Sebastian, Spain

 

Associate professor Rosario Sanchez-Pernaute

http://www.inbiomed.org/Index.php/en/lineas_de_investigacion/enfermedades_neurodegenerativas/laboratorio_celulas_madre_y_reparacion_neural

 

 

Associate professor Stefania Corti, IRCC Foundation Ca'Granda Ospedale Maggiore Policlinico, Milan, Italy

Stefania Corti

http://users.unimi.it/neuralstemlab/NeurostemMember.htm

 

Professor Rickard Sandberg, Karolinska Institutet, Stockholm, Sweden

 

Professor Rickard Sandberg (principal investigator)

http://sandberg.cmb.ki.se

 

 

Professor Caroline Graff, Karolinska Institutet, Stockholm, Sweden

 

Professor Caroline Graff

(photo:Stefan Zimmerman)

http://ki.se/en/nvs/caroline-graff-group

www.ki.se/brainbank